Enzymes Practice Questions

Question 1

4 marks

Sucrase is an enzyme found in the small intestine. It breaks down sucrose into glucose and fructose.

(a)

Which term describes sucrose in this reaction?

Put a cross ( ☒ ) in the box next to your answer.

☐ A active site

☐ B enzyme

☐ C product

☐ D substrate

(b)

Name the part of the sucrase molecule that sucrose binds to.

(c)

Sucrase cannot break down lactose.

Explain why.

Question 2

3 marks

Enzymes in the digestive system break down large food molecules into small soluble molecules.

(a)

Trypsin is a protease.

Which substances are produced when trypsin digests its substrate?

Put a cross ( ☒ ) in the box next to your answer.

☐ A sugars

☐ B amino acids

☐ C fatty acids

☐ D starches

(b)

Lipase digests the lipid in food.

Name the two products of this reaction.

Question 3

3 marks

Celery contains the enzyme catalase, which breaks down hydrogen peroxide into oxygen and water.

A student added the same mass of celery to hydrogen peroxide solution at different temperatures and measured the volume of oxygen produced in 60 seconds.

Temperature (°C) Volume of oxygen produced in 60 s (cm³)
10 4
20 9
30 17
40 24
50 11
60 0
(a)

State the optimum temperature for catalase shown by these results.

(b)

Explain why no oxygen was produced at 60 °C.

Question 4

3 marks

Milk is cloudy because it contains protein. When a protease digests this protein the milk becomes clear.

A student added protease solutions of different concentrations to milk and measured the time taken for the milk to become clear. The student calculated the rate of reaction using rate=1time in seconds\text{rate}=\frac{1}{\text{time in seconds}}.

Protease concentration (%) Time for milk to become clear (s) Rate (s−1\mathrm{s^{-1}})
0.5 400 0.003
1.0 250 0.004
1.5 140
2.0 80 0.013
(a)

Calculate the rate of reaction for the 1.5% protease solution. Give your answer to three decimal places.

.............................................................. s−1\mathrm{s^{-1}}

(b)

Name the dependent variable in this investigation.