Core Practical: Enzymes & pH Flashcards

All 4 cards in this deck

In the enzymes and pH core practical, what colour change shows that starch is still present?

Iodine solution turns blue-black.

Name two variables that must be controlled in an investigation into the effect of pH on enzyme activity.

Temperature and the concentration/volume of the solutions used.

How can the temperature be kept constant in an enzyme practical?

Use a water bath (or incubator) set to the required temperature.

In the enzymes and pH core practical, how do you know when all the starch has been broken down?

A drop of the mixture no longer turns the iodine blue-black – the iodine stays orange-brown. The time taken to reach this point is recorded.