Interpreting Chromatograms Flashcards

All 6 cards in this deck

In paper chromatography, what are the mobile phase and the stationary phase?

Mobile phase = the solvent running through the paper; stationary phase = the paper.

Why do the substances in a mixture move different distances up chromatography paper?

They have different solubilities in the solvent, so they move at different rates over the paper.

What does a chromatogram show for a pure substance?

A single spot. An impure substance (mixture) gives two or more spots.

What is the formula for the RfR_f value?

Rf=distance moved by the spotdistance moved by the solvent frontR_f = \frac{\text{distance moved by the spot}}{\text{distance moved by the solvent front}}
Rearranged: distance moved by spot =Rf×= R_f \times distance moved by solvent front.

A spot from a mixture has the same RfR_f value as a known pure substance run in the same solvent. What does this suggest?

The mixture probably contains that substance.

In paper chromatography, which substance in a mixture ends up furthest up the paper?

The one that is most soluble in the solvent. It travels with the moving solvent for more of the time, so it gets furthest from the start line.